فهرست:
فصل اول: مقدمه و کلیات
1-1- اهمیت محصولات کشاورزی در تأمین سلامتی انسان....................................................................................................................... 1
1-2- منشا و تاریخچه انگور.............................................................................................................................................................................. 1
1-3- مشخصات گیاه شناسی انگور................................................................................................................................................................... 2
1-4- رقم ریش بابا قرمز...................................................................................................................................................................................... 2
1-5- ارزش غذایی و اهمیت اقتصادی انگور................................................................................................................................................... 2
1-6- میزان تولید انگور در جهان...................................................................................................................................................................... 4
1-7- سطح زیر کشت انگور در ایران................................................................................................................................................................ 4
1-8- آناتومی حبه انگور...................................................................................................................................................................................... 5
1-9- دوره های رشد و رسیدن انگور................................................................................................................................................................ 6
1-9-1- مرحله اول: رشد سریع حبه................................................................................................................................................................ 6
1-9-2- مرحله دوم: توقف در رشد حبه.......................................................................................................................................................... 7
1-9-3- مرحله سوم: رسیدن میوه.................................................................................................................................................................... 7
1-10- رسیدن انگور و معیار آن........................................................................................................................................................................ 7
1-11- هدف از انجام تحقیق.............................................................................................................................................................................. 8
فصل دوم: بررسی منابع
2- 1- اهمیت کاربرد تکنیک حلقه برداری و اتفن........................................................................................................................................ 9
2- 2- حلقه برداری............................................................................................................................................................................................ 10
2- 2- 1- نحوه حلقه برداری........................................................................................................................................................................... 11
2-2- 2- اهداف حلقه برداری.......................................................................................................................................................................... 12
2- 2- 2- 1- افزایش تشکیل میوه............................................................................................................................................................... 12
صفحه فهرست مطالب عنوان
2- 2- 2- 2- افزایش اندازه حبه................................................................................................................................................................... 12
2- 2- 2- 3- زودرسی و رنگ گیری حبه ها............................................................................................................................................. 13
2- 2- 3- افزایش مواد فتوسنتزی در اثر حلقه برداری............................................................................................................................. 13
2- 2- 4- حلقه برداری و ترکیبات فنولی و آنتی اکسیدانی..................................................................................................................... 14
2- 2- 5- اثرات تضعیف کنندگی حلقه برداری......................................................................................................................................... 15
2- 2- 6- بستن قاعده شاخه های سبز سال جاری.................................................................................................................................. 15
2- 3- اسیدهای آلی در حبه انگور................................................................................................................................................................. 16
2- 3- 1- متابولیسم تارتاریک اسید.............................................................................................................................................................. 16
2- 3- 2- متابولیسم مالیک اسید................................................................................................................................................................... 17
2- 4- اتیلن و تاریخچه اتیلن در زیست شناسی گیاهی............................................................................................................................ 20
2- 4- 1- اتیلن ................................................................................................................................................................................................ 21
2- 4- 2- اتیلن و کیفیت میوه...................................................................................................................................................................... 21
2- 4- 3- بیوسنتز اتیلن: مکانیسم و تنظیم............................................................................................................................................... 22
2- 4- 4- مکانیسم سنتز اتیلن..................................................................................................................................................................... 22
2- 4- 5- اثرات مفید اتیلن............................................................................................................................................................................ 24
2- 4- 6- اثرات زیان آور اتیلن...................................................................................................................................................................... 25
2- 4- 7- اثرات اتیلن روی ظاهر میوه......................................................................................................................................................... 25
2- 4- 8- اثرات اتیلن روی بافت میوه......................................................................................................................................................... 25
2- 4- 9- تأثیر اتیلن روی عطر و طعم میوه................................................................................................................................................ 26
2- 4- 10- اثرات اتیلن روی فنول کل و ظرفیت آنتی اکسیدانی......................................................................................................... 26
2- 5- اتفن......................................................................................................................................................................................................... 27
صفحه فهرست مطالب عنوان
2- 6- سنتز فلاونوئید در انگور....................................................................................................................................................................... 28
2- 6- 1- تجمع فلاونوئیدها در حبه انگور.................................................................................................................................................. 29
2- 6- 2- تجمع فلاونوئید در بذر انگور....................................................................................................................................................... 30
2- 6- 3- مسیر بیوسنتز فلاونوئید در انگور .............................................................................................................................................. 31
فصل سوم: مواد و روش ها
3- 1- ماده گیاهی و تیمار حلقه برداری....................................................................................................................................................... 34
3- 2- تیمار اتفن................................................................................................................................................................................................ 34
3- 3- طرح آزمایشی......................................................................................................................................................................................... 34
3- 4- آزمونهای کیفی میوه........................................................................................................................................................................... 35
3- 4-1- اندازهگیری مواد جامد قابل حل کل عصاره میوه (TSS)....................................................................................................... 35
3- 4-2- اندازهگیری اسیدهای قابل تیتراسیون (TA)............................................................................................................................. 35
3- 4-3- اندازه گیری ضریب رسیدگی میوه (TSS/TA)...................................................................................................................... 36
3- 4-4- اندازه گیری pH آب میوه.............................................................................................................................................................. 36
3- 4- 5- تعیین سفتی بافت میوه................................................................................................................................................................. 36
3- 4- 6- اندازه گیری رنگ میوه.................................................................................................................................................................... 37
3- 4- 7- اندازه گیری محتوای فنل کل..................................................................................................................................................... 37
3- 4- 7- 1- تهیه محلول کربنات سدیم..................................................................................................................................................... 38
3- 4- 7- 2- رسم منحنی استاندارد اسید گالیک..................................................................................................................................... 38
3- 4- 8- تعیین میزان فعالیت آنتی اکسیدان کل عصاره میوه.............................................................................................................. 39
3- 4- 9- اندازه گیری ریزش حبه................................................................................................................................................................. 40
3- 4- 10- اندازه گیری طول و عرض خوشه.............................................................................................................................................. 40
صفحه فهرست مطالب عنوان
3- 4- 11- اندازه گیری وزن بیست حبه و خوشه..................................................................................................................................... 40
3- 5- تجزیه و تحلیل دادهها.......................................................................................................................................................................... 40
فصل چهارم: نتایج
4- 1- نتایج حلقه برداری و محلول پاشی اتفن و اثر متقابل آنها بر صفات کمی و کیفی اندازه گیری شده میوه انگور ریش بابا قرمز در زمان برداشت........................................................................................................................................................................................ 41
4- 2- مواد جامد محلول.................................................................................................................................................................................. 43
4- 3- اسیدهای قابل تیتراسیون.................................................................................................................................................................... 43
4- 4- ضریب رسیدگی میوه............................................................................................................................................................................ 44
4- 5- pH آب میوه.......................................................................................................................................................................................... 45
4- 6- طول خوشه.............................................................................................................................................................................................. 45
4- 7- عرض خوشه............................................................................................................................................................................................ 45
4- 8- وزن 20 حبه........................................................................................................................................................................................... 46
4- 9- وزن خوشه............................................................................................................................................................................................... 46
4- 10- ریزش حبه............................................................................................................................................................................................ 47
4- 11- سفتی بافت حبه...................................................................................................................................................................................48
4- 12- ترکیبات فنولی کل............................................................................................................................................................................. 49
4- 13- آنتی اکسیدان کل............................................................................................................................................................................... 49
4- 14- تغییرات رنگ میوه.............................................................................................................................................................................. 50
فصل پنجم: بحث
5- 1- مواد جامد محلول،اسیدیته کل، pH آب میوه و ضریب رسیدگی.............................................................................................. 53
5- 2- طول و عرض خوشه............................................................................................................................................................................... 55
صفحه فهرست مطالب عنوان
5- 3- وزن بیست حبه و خوشه...................................................................................................................................................................... 55
5- 4- ریزش حبه و سفتی بافت..................................................................................................................................................................... 56
5- 5- ترکیبات فنولی و آنتی اکسیدان کل................................................................................................................................................. 58
5- 6- رنگ میوه................................................................................................................................................................................................. 60
5- 7- نتیجه گیری کلی................................................................................................................................................................................... 62
پیشنهادات............................................................................................................................................................................................................ 63
فهرست منابع.......................................................................................................................................................................................................
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