فهرست:
فصل اول : کلیات تحقیق
1-1- تعریف فساد مواد غذایی...............................................................................................................................................2
1-1-1- فساد میکروبی............................................................................................................................................................2
1-1-1-1- تغییردر ترکیبات آلی غیر نیتروژنی.........................................................................................................................2
1-1-1-2- تغییر در ترکیبات آلی غیر نیتروژنی........................................................................................................................2
1-1-1-3- اسیدهای آلی..........................................................................................................................................................3
1-1-1-4-لیپیدها ....................................................................................................................................................................3
1-1- 2- فسادشیمیایی............................................................................................................................................................4
1-1-2- 1- فساد اکسیداتیو......................................................................................................................................................4
1-1-2-2- مراحل اکسیداسیون و مواد حاصل از آنها...............................................................................................................6
1-1-2-3- تشکیل هیدروپراکسید.............................................................................................................................................8
1-1-2-4- تجزیه هیدرو پراکسید............................................................................................................................................9
1-2-5- عوامل موثر در اکسیداسیون چربی ها و روغن ها.......................................................................................................9
1-2-5-1- ترکیب ماده غذایی ...............................................................................................................................................10
1-2-5-2- درجه حرارت.......................................................................................................................................................10
1-2-5-3- نور.......................................................................................................................................................................10 1-2-5-4- اکسیژن.................................................................................................................................................................10
1-2-5-5- رطوبت.................................................................................................................................................................10
1-2-5-6- کاتالیزورها............................................................................................................................................................10
1-3- روش های اندازه گیری پایداری روغن ها و چربی ها..............................................................................................11 1-3-1- اندا زه گیری مقاومت چربی و روغن در شرایط معمولی..........................................................................................12
1-3-2- آزمایش در گرمخانه .................................................................................................................................................12
1-3-3- ایندکس پایداری روغن............................................................................................................................................12
1-3-4- عدد پراکسید.............................................................................................................................................................13
1-3-5- عدد پارا آنیزین.........................................................................................................................................................13
1-3-6- آزمایش اسید تیوباربیتوریک......................................................................................................................................13
1-3-7- افزایش وزن..............................................................................................................................................................14
1-3-8- دی ان ها کونژوگه شده............................................................................................................................................14
1-3-9- اندازه گیری گاز در فضای بالای قوطی....................................................................................................................14
1-3-10- آنالیز حرارتی افتراقی..............................................................................................................................................14
1-3-11- رانسی مت..............................................................................................................................................................15
1-4- فاکتور حفاظت.............................................................................................................................................................15
1-5-آنتی اکسیدان ها............................................................................................................................................................16
1-5-1- آنتی اکسیدان های طبیعی..........................................................................................................................................17
1-5-2- آنتی اکسیدان های سنتتیک.......................................................................................................................................18
1-6- معایب آنتی اکسیدان های سنتتیک..............................................................................................................................19
1-7- تاریخچه و مقدمه درباره گل میمونی ........................................................................................................................20
1-7-1- گیاه شناسی گل میمونی............................................................................................................................................20
1-8- ماهی و نقش آن در تغذیه انسان.................................................................................................................................23
1-9- ماهی قزل آلای رنگین کمان.......................................................................................................................................24
1-10- فساد ماهی ها.............................................................................................................................................................24
1-10-1-عوامل موثر بر نوع و شدت فساد.............................................................................................................................25
1-10-1-1- نوع ماهی............................................................................................................................................................25
1-10-1-2- سن و شرایط ماهی به هنگام صید......................................................................................................................25
1-10-1-3- نوع و مقدارآلودگی گوشت ماهی به باکتریها.....................................................................................................25
1-10-1-4- درجه حرارت.....................................................................................................................................................25
1-10-2- علائم فساد درگوشت ماهی....................................................................................................................................25
1-10-3- فساد میکروبی ماهی................................................................................................................................................26
1-10-3-1- باکتری های عامل فساد......................................................................................................................................26
1-10-3-2- فساد ویژه ماهیان و فراورده های دریایی............................................................................................................26
1-10-4- فسادشیمیایی...........................................................................................................................................................26
1-10-4-1- اکسیداسیون چربی ها.........................................................................................................................................26
1-10-4-2- تغییرات پروتئینی................................................................................................................................................27
1-11- هدف.........................................................................................................................................................................28
فصل دوم : مروری بر پژوهشهای اخیر.....................................................................................................................29
فصل سوم : مواد و روش کار........................................................................................................................................32
3-1- منابع و تجهیزات و مواد مورد استفاده در مطالعه...........................................................................................................32
3-2- روش کار.......................................................................................................................................................................35
3-2-1- تهیه عصاره................................................................................................................................................................35
3-2-2- آماده سازی نمونه ماهی............................................................................................................................................35
3-3- آزمایش میکروبی (توتال کانت)....................................................................................................................................35
3-4- آزمایشات شیمیایی.......................................................................................................................................................36
3-4-1- اندازه گیری pH........................................................................................................................................................36
3-4-2- استخراج چربی و تعیین عدد پراکسید.......................................................................................................................36
3-4-3- تست عدد TBARS..................................................................................................................................................37
3-4-4- تعیین ازت پایه فرار کل TVB-N.............................................................................................................................38
3-5- ارزیابی حسی ...............................................................................................................................................................38
3-6- آزمون های آماری مورد استفاده در تحلیل نتایج حاصل از آزمایشات...........................................................................39
فصل چهارم : نتایج
4-1- آزمایش میکروبی (توتال کانت)....................................................................................................................................40
4-2- تعیین pH......................................................................................................................................................................41
4-3- تعیین عدد پراکسید و TBARS..................................................................................................................................42
4-4- عدد TVN...................................................................................................................................................................44
4-5- ارزیابی شاخص های حسی..........................................................................................................................................44
فصل پنجم : بحث و نتیجه گیری..................................................................................................................................46
5-1- آزمایش میکروبی(توتال کانت)......................................................................................................................................46
5-2- ارزیابی میزان pH..........................................................................................................................................................48
5-3- ارزیابی میزان عدد پراکسید PV وTBARS .............................................................................................................48
5-4- ارزیابی میزان TVN.....................................................................................................................................................50
5-5- ارزیابی شاخص های حسی...........................................................................................................................................51
-نتیجه گیری کلی و پیشنهادات....................................................................................................................................52
- فهرست منابع .................................................................................................................................................................53
- چکیده انگلیسی
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