فهرست:
فصل اول: مقدمه و بررسی منابع. 1
1-1- سرطان ریه. 2
1-1-1- عوامل خطرساز 3
1-1-2- تغییرات ژنی عامل سرطان ریه. 4
1-2- اهمیت شناسایی سرطان ریه. 5
1-3- روشهای شناسایی سرطان ریه. 6
1-3-1- نانوسیم‌های سیلیکا 7
1-3-2- نانوذرات طلا.. 10
1-3-3- نانولوله‌های کربنی.. 13
1-3-4- نقاط کوانتومی.. 17
1-4-گرافن. 21
1-5-گرافن اکسید. 24
1-6-کاربردهای گرافن اکسید. 27
1-6-1-کاربرد گرافن اکسید در بیوالکتروشیمی.. 28
1-6-2- کاربردهای پزشکی و زیستی گرافن اکسید. 29
1-7- هدف از کار پزوهشی حاضر. 38
فصل دوم: بخش تجربی.. 39
2-1- مواد و دستگاه‌ها 40
2-2- تهیه‌ی بافر Tris-HCl 42
2-3- سنتز گرافن اکسید. 42
2-4 آماده‌سازی محلول‌‌ها برای اندازه‌گیری طیف فلوئورسانس... 43
2-4-1- تهیه‌ی محلول مرحله‌ی اول. 43
2-4-2- تهیه‌ی محلول مرحله‌ی دوم. 43
2-4-3- تهیه‌ی محلول‌های مرحله‌ی سوم. 44
2-4-4- تهیه‌ی محلول مرحله‌ی چهارم. 44
2-4-5- تهیه‌ی محلول‌های مرحله‌ی پنجم.. 44
2-4-6- تهیه‌ی محلول‌های مرحله‌ی ششم.. 45
فصل سوم: نتایج و بحث.. 46
3-1- تهیه گرافن اکسید از گرافیت.. 47
3-2- بررسی طیف UV-Vis گرافن اکسید. 48
3-3- تفسیر طیف IR گرافن اکسید. 49
3-4- بررسی تصویر TEM گرافن اکسید. 49
3-5- انتخاب بیومارکر سرطان ریه. 50
3-6- تفسیر طیف‌های نشری. 53
3-6-1- بررسی طیف فلوئورسانس DNA پروب.. 53
3-6-2- بهینه‌سازی زمان جذب DNA پروب بر سطح GO.. 54
3-6-3- بهینه‌سازی مقدار GO در حضور DNA پروب.. 56
3-6-4- بررسی طیف فلوئورسانس کمپلکس DNA-GO پروب در حضور DNA هدف (DNA سالم) 57
3-6-5- بهینه‌سازی زمان هیبرید شدن DNA هدف با DNA پروب در حضور GO.. 58
3-6-6- بررسی تغییرات شدت فلوئورسانس کمپلکس DNA-GO پروب در حضور غلظت‌های مختلف DNA هدف 60
3-6-7- بررسی طیف فلوئورسانس DNA-GO پروب در حضور mDNA (DNA جهش‌دار) 62
3-7- شناسایی سرطان ریه. 63
3-8- نتیجه‌گیری. 65
3-9- پیشنهادات.. 66
منابع 67
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